Question

In E.Coli cloning vector pBR322, antibiotic resistance genes ($amp^R$) indicate which restriction sites?

• A. Pvul, Pstl
• B. PvulI, EcoRI
• C. BamHI, SaII
• D. EcoRI, HindRIII

Answer 1

Answer: (A)

Pvul, Pstl

Answer 2

The correct answer is option (A): Pvul, Pstl
pBR322 is a cloning vector that was constructed by Bolivar and Rodriguez. The structure of pBR322 has an origin of replication, restriction enzyme sites, and selectable marker sites. pBR322, have two antibiotic resistance genes, ampicillin and tetracycline. They are used as selectable markers. There are multiple restriction sites. In the tetracycline resistance gene, there are sites for BamHI and SalI. In the ampicillin resistance gene, there are sites for PstI and PvuI. There are other EcoRi, HindIII, and ClaI, that do not lie in any of the antibiotic resistance genes regions. In the ROP region, there is a site for PvuII. Therefore, the correct answer is PstI and PvuI.