Question

In $DNA$ fingerprinting, the process of $DNA$ hybridisation with the help of specific $DNA$ probe is described as

• A. Western blotting
• B. Northern blotting
• C. Southern blotting
• D. All of the above

Answer 1

Answer: (C)

Southern blotting

Answer 2

Edward Southern in $1975$ first developed the technique Southern blotting. It is used in DNA fingerprinting. The probes or markers that are radioactive synthetic DNA segments target a specific nucleotide sequence which is complementary to VNTRs sequences and hybridises them.