Question
Question: In chromatography the absorbent medium eg. Whatman filter paper is known as the stationary phase. Ex...
In chromatography the absorbent medium eg. Whatman filter paper is known as the stationary phase. Explain.
Solution
Chromatography is a process used to separate the components of a mixture. It has 2 phases: the mobile phase and the stationary phase. The mobile phase is observed in the stationary phase.
Complete step by step answer:
-Chromatography is a technique used for separating the mixture. The solution or the mixture is passed through a medium in which the components move at a different rate or different speed.
-This technique is used for separation and the separation is based on the solubility of the compounds.
-This technique consists of two phases; the mobile phase and the stationary phase.
Mobile phase: The mixture which is dissolved in the fluid is called the mobile phase. It is carried through a structure that holds another medium called the stationary phase.
-Stationary phase: It is called the absorbent medium. It can be liquid, gel, or even solid. It remains immovable when a gas or a liquid moves over the adsorbent surface. It remains stationary throughout the process. Examples of stationary phases are filter paper, glass, silica, Whatman filter paper, etc.
-Whatman filter paper: It consists of 99% of alpha-cellulose. It is mainly cellulose fiber and is a polymer. The polymer chain consists of a functional group OH which sticks to the polymer chain. The cellulose in the paper acts as water absorbing material which acts as a stationary phase.
Hence, the absorbent medium eg. Whatman filter paper is known as the stationary phase in chromatography.
Note
There are 4 types of chromatography: Gas chromatography, Liquid chromatography, Thin-layer chromatography (TLC), and Paper chromatography. For testing the water samples we use Liquid chromatography.