Question

In a polymerase chain reactions, temperatures required for the steps

(i) Denaturations,

(ii) Annealing and

(iii) Extension are respectively

• A. (i) $40{^\circ}C$ (ii) $72{^\circ}C$ (iii) $94{^\circ}C$
• B. (i) $94{^\circ}C$ (ii) $72{^\circ}C$ (iii) $40{^\circ}C ⥂$
• C. (i) $72{^\circ}C$ (ii) $94{^\circ}C$ (iii) $40{^\circ}C ⥂$
• D. (i) $94{^\circ}C$ (ii) $40{^\circ}C$ (iii) $72{^\circ}C$

Answer 1

Answer: (D)

(i) $94{^\circ}C$ (ii) $40{^\circ}C$ (iii) $72{^\circ}C$