Question
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Nitrous acid (HNO2) converts amino acids into hydroxy acids with retention of configuration. Estimation of nitrogen gas evolved in the reaction is the basis of Van slyke estimation of amino acids.
Which of the following amino acids cannot be analysed by Van slyke method?
I – 
(cysteine)
II – 
(Proline)
III – 
(Histidine)
IV – 
(Valine)
A
Only I
B
Only II
C
I and III
D
I, III, IV
Answer
Only II
Explanation
Solution
Since proline has 2º amino group.