Question
Question: If a nucleotide lacking a hydroxyl group at the 3’ end is added to a PCR, what would be the outcome?...
If a nucleotide lacking a hydroxyl group at the 3’ end is added to a PCR, what would be the outcome?
A
No additional nucleotides would be added to a growing strand containing that nucleotide.
B
Strand elongation would proceed as normal.
C
Nucleotides would only be added at the 5’ end.
D
T. aquaticus DNA polymerase would be denatured.
Answer
No additional nucleotides would be added to a growing strand containing that nucleotide.