Question
Biology Question on Expression vectors
Identify the missing restriction sites (A, B, and C) in the given E. coli cloning vector pBR322.
A
A - BamHI; B - ClaI; C - PstI
B
A - PstI; B - ClaI; C - HindII
C
A - BamHI; B - HindIII; C - ClaI
D
A - ClaI; B - PstI; C - BamHI
Answer
A - BamHI; B - ClaI; C - PstI
Explanation
Solution
In the pBR322 vector, the correct restriction sites are: A - BamHI, B - ClaI, and C - PstI. These sites are strategically placed to allow easy insertion and modification within the antibiotic resistance regions of pBR322.