Question

Hybridoma technology was developed by?

Answer 1

Hybridoma technology could be used for the assembly of huge numbers of identical antibodies also called monoclonal antibodies. The myeloma cell line that's utilized during this process is chosen for its ability to grow in tissue culture and for an absence of antibody synthesis.

Complete answer:
Hybridoma technology was also discovered in 1975 by two great scientists, Georges Kohler and Cesar Milstein. They wanted to form the immortal hybrid cells by fusing normal B cells from immunized mice with their respective myeloma cells. Hybridoma technology could even be used for producing large numbers of identical antibodies.

This process starts by injecting a mouse with an antigen that provokes an immune response . a sort of white blood cell, the B cell, produces antibodies that bind to the injected antigen. The antibody which are producing B-cells are then harvested from the mouse and, in turn, after fused with the immortal B cell cancer cells, myeloma, to provide a hybrid cell line called a hybridoma, which has both the antibody-producing ability of the B-cell and hence the longevity and reproductivity of the myeloma.

Hybridoma technology was discovered in 1975 by two scientists, Georges Kohler and Cesar Milstein. They wanted to make immortal hybrid cells by fusing normal B cells from immunized mice with their myeloma cells. For incidental reasons, they had all the wants fulfilled and it worked within the first attempt.

Note: The myeloma cell line that's utilized during this process is chosen for its ability to grow in tissue culture and for an absence of antibody synthesis. In contrast to polyclonal antibodies, which are mixtures of numerous different antibody molecules, the monoclonal antibodies produced by each hybridoma line are all chemically identical.