Question

How was insulin obtained before the advent of rDNA technology? What were the problems encountered?

Answer 1

Insulin is a hormone produced by your pancreas that regulates the quantity of glucose in your bloodstream. It also aids glucose storage in the liver, fat, and muscles. Finally, it controls the metabolism of carbs, lipids, and proteins in your body. Insulin is made by cells called islets of Langerhans in the pancreas. These cells continuously release a little quantity of insulin into the body, but as blood glucose levels rise, they release large amounts of the hormone.

Complete answer:
Insulin was formerly extracted and refined from the pancreas of pigs and cows in order to treat diabetes. Despite its effectiveness, animal insulin provoked allergic reactions in a few patients due to slight structural alterations.
Human insulin differs slightly from insulin obtained from slaughtered cattle and pigs. It has long-term negative consequences.

Additional information:
If your blood glucose level is abnormally high when you're diagnosed with type two diabetes, your doctor may recommend that you use insulin to lower it- a much faster method than diet and exercise
Insulin may be used for a variety of reasons by patients with type two diabetes:
It can swiftly lower your blood glucose level to a more manageable level.
It has fewer negative effects than some medications:
It may be less expensive.

Note:
One of the first biotech products was recombinant human insulin. It was created in response to the necessity for a reliable and sufficient supply all across the world. Animal insulins and semisynthetic insulins made by modifying animal insulins were superseded by recombinant human insulin.
Recombinant DNA Technology's Limitations. Native species are wiped out in the environments where genetically modified organisms are introduced. Resistant plants can possibly produce resistant weeds that are difficult to control. Between-organism cross-contamination and movement of proprietary DNA.