Question

How can DNA be extracted from Saliva?

Answer 1

Saliva is an extracellular fluid that the salivary glands in the mouth generate and discharge. Saliva contains 98 percent water plus electrolytes, mucus, white blood cells, epithelial cells, enzymes, antibacterial compounds such as secretory IgA, and lysozymes in humans.

Complete answer:
Several processes are required for DNA extraction from saliva:
a) Collection and storage,
b) Cell lysis,
c) RNase treatment,
d) Protein precipitation,
e) Ethanol precipitation, and
f) DNA rehydration.

The previously described2 DNA Stabilization Buffer solution performs properly without modification. There was no attempt to optimise the RNase treatment and DNA rehydration processes. Several factors that might impact yield were found for each remaining phase. Each variable was individually tweaked, and the improvement in yield and quality was statistically analysed. The ideal values for variables that were proved to increase yield and/or DNA quality were incorporated in the final procedure.

Note: Deoxyribonucleic acid is a molecule made up of two polynucleotide chains that coil around each other to create a double helix that contains genetic sequence, maintenance, development, and replication of all kinds of organisms and many pathogens. DNA and ribonucleic acid are both nucleic acids.