Question

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements, choose the correct answer from the options given below:

• A. Both (A) and (R) are correct and (R) is the correct explanation of (A)
• B. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
• C. (A) is correct but (R) is not correct
• D. (A) is not correct but (R) is correct

Answer 1

Answer: (B)

Both (A) and (R) are correct but (R) is not the correct explanation of (A)

Answer 2

PCR, a technique used for amplifying DNA, does not involve the use of selectable markers. In contrast, selectable markers, such as antibiotic resistance genes, are typically employed during transformation procedures to aid in the selection of cells that have taken up the foreign DNA.