Question

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement

• A. The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
• B. The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
• C. The enzyme cuts DNA molecule at identified position within the DNA.
• D. The enzyme binds DNA at specific sites and cuts only one of the two strands

Answer 1

Answer: (D)

The enzyme binds DNA at specific sites and cuts only one of the two strands

Answer 2

Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbone.