Question

Following are the steps of southern blot procedure.

I. Autoradiography

II. Hybridization with radioactive nucleic acid (probe)

III. Blotting

IV. DNA fragments are treated to make them single stranded

V. Electrophoresis

VI. Clearing of DNA by restriction endonuclease

VII. Isolation of DNA from smaple

The correct sequence is -

• A. 1, 2, 3, 4, 5, 6, 7
• B. 7, 6, 5, 4, 3, 2, 1
• C. 1, 2, 6, 7, 3, 4, 5
• D. 7, 6, 1, 2, 5, 3, 4

Answer 1

Answer: (B)

7, 6, 5, 4, 3, 2, 1