Question

Following are the steps involved in the process of PCR.
a. Annealing
b. Amplification (~1 billion times)
c. Denaturation
d. Treatment with Taq polymerase and deoxynucleotides
e. Extension
Choose the correct sequence of steps of PCR from the options given below :

• A. c → a → d → e → b
• B. a → b → e → d → c
• C. a → c → e → d → b
• D. d → b → e → c → a

Answer 1

Answer: (A)

c → a → d → e → b

Answer 2

The correct sequence of steps in PCR is: C. Denaturation A. Annealing D. Treatment with Taq polymerase and deoxynucleotides E. Extension B. Amplification ( 1 billion times)
Thus, C → A → D → E → B