Question

Fill up the blanks –

When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of _______ and these can be joined together end-to-end using

Disruption of the cell membranes can be achieved by treating the bacterial cells plant or animal tissue with enzymes such as ______ (bacteria), ______ (plant cells) and _____ (fungus). If any protein encoding gene is expressed in a heterologous host, it is called a ______.In gel electrophoresis, the separated DNA fragments can be seen only after staining the DNA with a compound known as _____ followed by exposure of UV radiation

• A. A – sticky ends, recombinase; B – lysozyme, cellulase, chitinase; C – recombinant protein; D – ethidium bromide
• B. A – sticky ends, DNA ligases; B – exonuclease, cellulase, chitinase; C – recombinant protein; D – ethidium bromide
• C. A – sticky ends, DNA ligases; B – lysozyme, cellulase, chitinase; C – native protein; D – cesium chloride
• D. A – sticky ends, DNA ligases; B – lysozyme, cellulase, chitinase; C – recombinant protein; D – ethidium bromide

Answer 1

Answer: (D)

A – sticky ends, DNA ligases; B – lysozyme, cellulase, chitinase; C – recombinant protein; D – ethidium bromide