Question: Experiments 1, 2, and 3, were conducted wherein synthetic vesicles containing F<sub>0</sub> –F<sub>1...

Question

Experiments 1, 2, and 3, were conducted wherein synthetic vesicles containing F₀ –F₁ ATP synthase were prepared and incubated overnight in a tube. Subsequently, the vesicles were transferred to another tube which also contained ADP and Pi (inorganic phosphate).

A. A proton gradient across the vesicular membrane will be present in both experiments 1 and 2 at the time of transfer.

B. As a consequence of the proton gradient, ATP will be synthesized in both experiments 1 and 2.

C. ATP will be synthesized in experiment 3 because Fo-F1 ATP synthase has the inherent property to catalyse the synthesis of ATP from ADP and Pi.

D. ATP will be synthesized in experiment 2 because the proton has to flow out of the vesicles through the F₀ –F₁ ATP synthesis for ATP synthesis.