Question
Question: Elution is the process for- A) Crystallization of compound B) Separation of compound C) Extr...
Elution is the process for-
A) Crystallization of compound
B) Separation of compound
C) Extraction of compound
D) Distillation of compound
Solution
It is used in chromatographic techniques.
Complete Solution :
We have to say whether the elution process is used for which of the following from the options.
- Elution is basically an analytical technique having organic chemistry as its background. It is an analytical technique which is incorporated in such cases where we have to extract one or more ions from a solution where there is a large number of ions.
- So elution is a process in which we extract the required ions or compounds from a solution. Elution process is mainly used in chromatographic techniques
- In column chromatography the analyte i.e. the substance we are analysis will be bound a stationary phase say like a resin, which is the adsorbent and a solvent or we say it as eluent is used to wash out the analyte from the resin.
Procedure of elution-
We take an appropriate eluent and are allowed to follow down the column (the adsorbate) and the liquid flowing down is collected and the time of flow is noted.
- Principle-
Here the solvent i.e. eluent flowing down the column has a tendency either to flow down the column passing the analyte-solute complex or it replaces the analyte from the adsorbent and flows down carrying the analyte. This is so due to the affinity of the analyte with the adsorbate. When the eluent is allowed to flow down the column the eluent tries to break the bond of the analyte attached with the resin and the analyte with least affinity flows down along with the eluent and with high affinity comes last and these are collected separately and is analyzed.
And we note the eluent volume and eluent time for the further calculation for the analyte.
So, the correct answer is “Option C”.
Note: We should use the eluent, which is appropriate for the requirement of the analyte. And we should use a noble adsorbate that is useful for the process of elution, which prevents the mixing of the adsorbate with the eluent.