Question
Question: ELISA is used to detect viruses where the key reagent is A)Alkaline phosphatase B)Catalase C)D...
ELISA is used to detect viruses where the key reagent is
A)Alkaline phosphatase
B)Catalase
C)DNA probe
D)RNase
Solution
A test that detects and analyses antibodies in your blood is an enzyme-linked immunosorbent assay, also known as ELISA or EIA. You may use this test to assess if you have antibodies that are linked to certain infectious conditions.
Complete answer:
The initial test for diagnosing AIDS is the ELISA (enzyme linked immunosorbent assay) screening test. The test works by identifying antibodies/substances or proteins that are produced when viruses are present in the blood. Alkaline phosphatase and peroxidases are widely used to conduct the ELISA test with enzymes as the main reagent.
These enzymes are used to supply a specialised ELISA plate or tray with an antibody-antigen complex. In an ELISA test to detect a specific antigen, the antibody is buffered and a drop of serum (centrifugal blood supernatant) is poured over it. If the latter contains antigen, the antigen-antibody complex will be formed.
An ELISA test may be used to diagnose:
1)HIV, which causes AIDS
2)Lyme disease
3)pernicious anemia
4)Rocky Mountain spotted fever
5)rotavirus
6)squamous cell carcinoma
7)syphilis
8)toxoplasmosis
9)varicella-zoster virus, which causes chickenpox and shingles
10)Zika virus
Before more in-depth trials are ordered, ELISA is also used as a screening method. If you have signs or symptoms of the above conditions, a doctor might recommend this test. If they want to rule out any of these conditions, your doctor can order this test as well.
Hence, the correct answer is option (A)
Note: There can be false positives and false negatives. A false-positive result means that if you really don't, you have a condition. A false-negative outcome means that when you really do, you do not have a condition. As a result, in a few weeks, you might be asked to repeat the ELISA again, or your doctor may order more sensitive tests to validate or disprove the findings.