Question

ELISA is used to detect viruses where the key reagent is
A. Alkaline phosphatase
B. Catalase
C. DNA probe
D. RNase

Answer 1

The enzyme-linked immunosorbent assay is generally called ELISA. It is a common laboratory technique, which is used to measure the concentration of an analyte that are antibodies or antigens in a given solution or sample. The purpose of an ELISA is to determine if a particular protein is present in a sample and how much in quantity.

Complete answer:
-The ELISA is also known as the Enzyme ImmunoAssay (EIA) has become a widely used serological technique for the detection of AIDS in the blood serum of HIV-infected persons. The alkaline phosphatase, β-galactosidase, lactoperoxidase, etc these are some enzymes used for labeling in the ELISA test.
-Catalase is considered one of the most important antioxidant enzymes. it decomposes hydrogen peroxide into water and oxygen. it is used as a therapeutic agent in many oxidative stress-related diseases.
-DNA probes are stretches of single-stranded DNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. Generally, radioisotopes, epitopes, or fluorophores are labeled to DNA probe to enable the target sequence detection.
-Ribonucleases are generally called RNase, these are a broad class of RNA-degrading enzymes that are possessed by all organisms, and these are extremely common in the environment. RNase cleaves RNA molecules at a specific recognition site, so the enzyme is commonly used in molecular biology for the removal of RNA within DNA and protein samples.
Hence, the correct answer is option A.

Note: Antibody coating, Specific capture antibody is immobilized on high protein-binding plates by overnight incubation, protein capture, samples, and standard dilutions are added to the wells and will be captured by the bound antibodies, detection antibody, streptavidin-enzyme conjugate, the addition of substrate, analysis.