Question

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

• A. Annealing
• B. Extension
• C. Denaturation
• D. Ligation

Answer 1

Answer: (C)

Denaturation

Answer 2

During $PCR$ process, if high temperature is not maintained, the initial step of the process known as denaturation of DNA is not done.