Question

During isolation of genetic material, the chemical used to precipitate out the purified $DNA$ is

• A. bromophenol blue
• B. chilled ethanol
• C. ethidium bromide
• D. both (a) and (c)

Answer 1

Answer: (B)

chilled ethanol

Answer 2

The purified $DNA$, after treatment with various enzymes, precipitates out after addition of chilled ethanol. This is viewed as a collection of fine threads in the suspension, and is easily collected. The process is known as $DNA$ spooling.