Question

$DNA$ is a polymer of nucleotides which are linked to each other by $3'-5'$ phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?

• A. Replace purines with pyrimidines
• B. Remove/Replace $3' OH$ group in deoxyribose
• C. Remove/Replace $2' OH$ group with some other group in deoxyribose
• D. Both (b) and (c)

Answer 1

Answer: (B)

Remove/Replace $3' OH$ group in deoxyribose

Answer 2

If $3' OH$ group is removed/replaced in deoxyribose, there will be no formation of phosphodiester bonds and hence polymerisation of nucleotides will be prevented.