Question
Question: Describe in short the various steps that occur during the photochemical phase of photosynthesis....
Describe in short the various steps that occur during the photochemical phase of photosynthesis.
Solution
The process of photosynthesis completes in two steps –
(i) Light Reaction and (ii) Dark Reaction
- Light Reaction is known as photochemical phase
- It occurs in Thylakoid
- Products produced in photochemical phase ATP, NADPH are utilised by the dark phase
Complete answer:
(a) Light Reaction or photochemical phase begins as soon as sunlight falls on green plants.
(b) Pigment molecules present in the photosystemIand photosystemIIabsorb light of specific wavelength.
(c) Accessory pigments absorb light and transfer energy to their reaction centres
(d) PS I and PS II are connected with each other by electron transport chain
(e) The electrons move in PS I and PS II through two pathways
(i) Non – cyclic and
(ii) Cyclic.
Non-cyclic photophosphorylation – it involve both PS I and PS{\text{ }}II$$$$ATP, NADPH$$$ + {H^ + }$ are synthesised by this electron flow. Firstly PSII, having ${P_{680}}$ molecules absorbs light, electrons gain energy from light and become excited and transfer energy to electron transport system cytochromes. This movement is downhill.
(f) The electrons then transport to the PSI $\left( {{P_{700}}} \right)$. These electrons then moved downhill again to a molecule of $NAD{P^ + }$. The addition of these electrons reduces the $NAD{P^ + }$ to $NADPH + {H^ + }$.
Cyclic photophosphorylation: In this process electrons released by the excited photo centre are returned to it after passing through a series of electron carriers. It involves only PSI. The excited electrons do not pass on to $NAD{P^ + }$ and are cyclic back to PSI.
Here only ATP synthesis takes place
It takes in stroma lamellae membranes.
The membrane or lamella of grana have both PSI and PS{\text{ }}II.ThestromalamellaemembranelacksPS{\text{ }}II$$ and NADP reductase enzyme.
Water splitting complex is associated with PSII. Water splits into H+,[O] and electrons. The protons and oxygen formed by splitting of water are released into the lumen of Thylakoid.
The oxygen produced is released as one of the net product of photosynthesis
2H2O→4H++O2+4e−
Note: Photochemical phase generates assimilatory power in the form of ATP and NADPH, which is used in carbohydrate synthesis in dark reaction.