Question

Complete the accompanying flow chart of DNA finger printing.

Isolation of DNA

Transferring of DNA to (3)

DNA hybridisation using (4)

\begin{tabular} { | l | } \hline Detecting of hybridised DNA by (5) \ \hline \end{tabular}

• A. 1 – Restriction endonuclease; 2 – Electrophoresis; 3 – Nitrocellulose or nylon; 4 – Labelled VNTR probe; 5 – Autoradiography
• B. 1 – Electrophoresis; 2 – Restriction endonuclease; 3 – Nitrocellulose or nylon; 4 – Labelled VNTR probe; 5 – Autoradiography
• C. 1 – Restriction endonuclease; 2 – Electrophoresis; 3 – Labelled VNTR probe; 4 – Nitrocellulose or nylon; 5 – Autoradiography
• D. 1 – Restriction endonuclease; 2 – Electrophoresis; 3 – Nitrocellulose or nylon; 4 – Autoradiography; 5 – Labelled VNTR probe

Answer 1

Answer: (A)

1 – Restriction endonuclease; 2 – Electrophoresis;

3 – Nitrocellulose or nylon; 4 – Labelled VNTR probe;

5 – Autoradiography