Question
Biology Question on biotechnology
Commonly used vectors for human genome sequencing are
A
T-DNA
B
BAC and YAC
C
expression vectors
D
T/A cloning vectors.
Answer
BAC and YAC
Explanation
Solution
Bacterial artificial chromosome (BAC) vectors are based on the natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker, and a cloning site.
- These vectors can accommodate up to 300-350 kb of foreign DNA and are also being used in genome sequencing projects.
- Yeast artificial chromosome (YAC) vectors are used to clone DNA fragments of more than 1 Mb in size.
- Therefore, they have been exploited extensively in mapping large genomes, e.g., in the Human Genome Project.
- These vectors contain the telomeric sequence, the centromere, and the autonomously replicating sequence from yeast chromosomes.
Discover More from Chapter: Genome and Genomics