Question
Biology Question on Structure of DNA and RNA
Cloning with lambda replacement vectors involves the following steps. Arrange them from first to last, in the order they are performed.
A. Packaging of DNA to form viable phage particles
B. Using DNA ligase to connect lambda fragments to foreign DNA
C. Isolating vector DNA from phage particles
D. Infecting E-coli cells, with recombinant phage
E. Cutting vector DNA with suitable restriction enzyme
Choose the correct answer from the options given below:
A
A, C, B, E, D
B
D, C, E, B, A
C
B, A, C, E, D
D
C, E, B, A, D
Answer
C, E, B, A, D
Explanation
Solution
The correct answer is (D) : C, E, B, A, D.