Question

Arrange the various steps of DNA fingerprinting technique in the correct order.

(i) Separation on DNA fragments by electrophoresis.

(ii) Digestion of DNA by restriction endonucleases.

(iii) Hybridization using labeled VNTR probe.

(iv) Isolation of DNA.

(v) Detection of hybridized DNA fragments by auto radio graphy.

(vi) Transferring the separated DNA fragments to nitro cellulose membrane.

• A.
• B. (ii) $\rightarrow$ (i) $\rightarrow$ (iv) $\rightarrow$ (vi) $\rightarrow$ (iii) $\rightarrow$ (v)
• C. (iv) $\rightarrow$ (ii) $\rightarrow$ (i) $\rightarrow$ (vi) $\rightarrow$ (iii) $\rightarrow$ (v)
• D.

Answer 1

Answer: (C)

(iv) $\rightarrow$ (ii) $\rightarrow$ (i) $\rightarrow$ (vi) $\rightarrow$ (iii) $\rightarrow$ (v)