Question
Biology Question on Molecular Biology
Arrange the given steps of DNA fingerprinting in the sequence from initiation to end:
(A) Digestion of DNA by restriction endonuclease
(B) Isolation of DNA
(C) Hybridisation using labelled VNTR probe
(D) Transferring (blotting) of separated DNA fragments to synthetic membrane
Choose the correct answer from the options given below:
A
(A), (B), (C), (D)
B
(A), (D), (C), (B)
C
(B), (A), (D), (C)
D
(C), (A), (B), (D)
Answer
(B), (A), (D), (C)
Explanation
Solution
The correct order of steps in DNA fingerprinting is:
(B) Isolation of DNA
(A) Digestion of DNA by restriction endonuclease
(D) Transferring (blotting) of separated DNA fragments to synthetic membrane
(C) Hybridisation using labelled VNTR probe.