Question

Arrange the following steps of PCR in correct sequence.
(A) Temperature induced denaturation of double stranded DNA.
(B) Isolation of gene of interest
(C) Amplification of gene by using Taq polymerase
(D) Primer annealing on the separated templates
Choose the correct answer from the options given below:

• A. (A), (C), (B), (D)
• B. (B), (A), (D), (C)
• C. (A), (B), (D), (C)
• D. (B), (A), (C), (D)

Answer 1

Answer: (B)

(B), (A), (D), (C)

Answer 2

The correct option is (B) :(B), (A), (D), (C)