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Biology Question on Applications of Biotechnology

Arrange the following steps of DNA fingerprinting in proper sequence:
(A) Hybridization using labeled VNTR probe
(B) Separation of DNA fragments by electrophoresis
(C) Digestion of DNA by restriction endonucleases
(D) Blotting of separated DNA fragments to nylon
(E) Isolation of DNA
Choose the correct answer from the options given below:

A

(C), (A), (B), (D), (E)

B

(E), (C), (B), (D), (A)

C

(B), (C), (D), (E), (A)

D

(C), (D), (B), (A), (E)

Answer

(E), (C), (B), (D), (A)

Explanation

Solution

The correct order for DNA fingerprinting is: (E) Isolation of DNA, (C) Digestion by restriction endonucleases, (B) Separation by electrophoresis, (D) Blotting to nylon membrane, and (A) Hybridization with a labeled probe. DNA fingerprinting is a powerful technique used for identification based on DNA variations.