Question
Biology Question on Transformation of Bacteria
Arrange the following steps involved in the transformation of bacteria in a sequence from initiation to end.
(A) Incubation of rDNA with bacterial cell on ice
(B) Treatment with divalent cations
(C) Heat shock treatment
(D) Selection on antibiotic containing agar plate
(E) Placed them again on ice
Choose the correct answer from the options given below:
A
(A), (B), (D), (C), (E)
B
(B), (A), (C), (E), (D)
C
(B), (C), (D), (A), (E)
D
(A), (C), (B), (D), (E)
Answer
(B), (A), (C), (E), (D)
Explanation
Solution
The correct sequence of transformation involves treating bacteria with divalent cations,incubating on ice, heat shock, and finally selecting on an antibiotic plate.