Question

Arrange the following steps in sequence of their order for production of recombinant Insulin:-
A. Fusion of A and B chains for disulphide bond.
B. Cynogen bromide treatment to remove methionine and â galactosidase.
C. Introduction of A and B chain in the plasmid containing â galactosidase gene.
D. Synthesis of A and B chain in E coil.

• A. a→ b→ d→ c
• B. d→c→a→ b
• C. c→d→b→a
• D. b→a→d→c

Answer 1

Answer: (C)

c→d→b→a

Answer 2

The correct answer is option (C) : c→d→b→a