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Question: Arrange the following steps in sequence for construction of artificial recombinant DNA: (1) Use of ...

Arrange the following steps in sequence for construction of artificial recombinant DNA:

(1) Use of restriction enzymes to isolate to required gene.

(2) Transfer of recombinant DNA into host.

(3) Multiplication of recombinant DNA.

(4) Required gene linked with plasmid DNA.

A

BDCAB \rightarrow D \rightarrow C \rightarrow A

B

BCDAB \rightarrow C \rightarrow D \rightarrow A

C

ABCDA \rightarrow B \rightarrow C \rightarrow D

D

ADBCA \rightarrow D \rightarrow B \rightarrow C

Answer

ADBCA \rightarrow D \rightarrow B \rightarrow C