Question
Biology Question on Recombinant DNA technology and applications
Arrange the following steps in recombinant DNA technology on the basis of their sequence of occurrence:
(A) Isolation of desired DNA fragments
(B) Culturing the host cells
(C) Extraction of desired product
(D) Ligation of DNA fragment into vector
(E) Transferring of recombinant DNA to host
Choose the correct answer from the options given below:
A
(A), (E), (C), (B), (D)
B
(A), (D), (E), (B), (C)
C
(B), (C), (A), (D), (E)
D
(B), (A), (D), (C), (E)
Answer
(A), (D), (E), (B), (C)
Explanation
Solution
The correct order is: (A) Isolation of desired DNA fragments, (D) Ligation of DNA fragment into vector, (E) Transferring recombinant DNA to host, (B) Culturing host cells, and (C) Extraction of the desired product. This sequence follows the typical recombinant DNA methodology.