Question

Arrange the following steps in a correct sequence as per Gram’s staining technique:

Treatment with 0.5% iodine solution (1), washing with water (2), treatment with absolute alcohol/acetone (3), staining with weak alkaline solution of crystal violet (4).

• A. 4 → 1 → 2 → 3
• B. 3 → 2 → 1 → 4
• C. 3 → 1 → 2 → 4
• D. 4 → 2 → 3 → 1

Answer 1

Answer: (A)

4 → 1 → 2 → 3